主要研究成果----膜生物学国家重点实验室

主要研究成果

Zheng, K.W., Zhang, J.Y., He, Y.D., Gong, J.Y., Wen, C.J., Chen,J.N., Hao, Y.H., Zhao, Y. and Tan, Z. (2020) Detection of genomic G-quadruplexes in living cells using a small artificial protein. Nucleic Acids Res., 48, 11706-11720.
Zheng, K.W., Liu, C., Meng, Q., Hao, Y.H., Zheng, J.P., Li, W. and Tan, Z. (2020) One-Step High-Throughput Telomerase Activity Measurement of Cell Populations, Single Cells, and Single-Enzyme Complexes. ACS Omega, 5, 24666-24673.
Zhang, J.Y., Xia, Y., Hao, Y.H. and Tan, Z. (2020) DNA:RNA hybrid G-quadruplex formation upstream of transcription start site. Sci Rep, 10, 7429.
Wen, C.J., Gong, J.Y., Zheng, K.W., He, Y.D., Zhang, J.Y., Hao, Y.H. and Tan, Z. (2020) Targeting nucleic acids with a G-triplex-to-G-quadruplex transformation and stabilization using a peptide-PNA G-tract conjugate. Chem. Commun. (Camb.), 56, 6567-6570.
He, Y.D., Zheng, K.W., Wen, C.J., Li, X.M., Gong, J.Y., Hao, Y.H., Zhao, Y. and Tan, Z. (2020) Selective Targeting of Guanine-Vacancy-Bearing G-Quadruplexes by G-Quartet Complementation and Stabilization with a Guanine-Peptide Conjugate. J. Am. Chem. Soc., 142, 11394-11403.
Zheng, K.W., Zhang, J.Y. and Tan, Z. (2019) Characterization of Co-transcriptional Formation of G-Quadruplexes in Double-Stranded DNA. Methods Mol. Biol., 2035, 243-255.
Xia, Y., Zheng, K.W., He, Y.D., Liu, H.H., Wen, C.J., Hao, Y.H. and Tan, Z. (2018) Transmission of dynamic supercoiling in linear and multi-way branched DNAs and its regulation revealed by a fluorescent G-quadruplex torsion sensor. Nucleic Acids Res., 46, 7418-7424.
Zheng, K.W., He, Y.D., Liu, H.H., Li, X.M., Hao, Y.H. and Tan, Z. (2017) Superhelicity Constrains a Localized and R-Loop-Dependent Formation of G-Quadruplexes at the Upstream Region of Transcription. ACS Chem. Biol., 12, 2609-2618.
Zhao, Y., Zhang, J.Y., Zhang, Z.Y., Tong, T.J., Hao, Y.H. and Tan, Z. (2017) Real-Time Detection Reveals Responsive Cotranscriptional Formation of Persistent Intramolecular DNA and Intermolecular DNA:RNA Hybrid G-Quadruplexes Stabilized by R-Loop. Anal. Chem., 89, 6036-6042.
Li, X.M., Zheng, K.W., Hao, Y.H. and Tan, Z. (2016) Exceptionally Selective and Tunable Sensing of Guanine Derivatives and Analogues by Structural Complementation in a G-Quadruplex. Angew. Chem. Int. Ed. Engl., 55, 13759-13764.
Liu, H.h., Zheng, K.w., He, Y.d., Chen, Q., Hao, Y.h. and Tan, Z. (2016) RNA G-quadruplex formation in defined sequence in living cells detected by bimolecular fluorescence complementation. Chem. Sci., 7, 4573-4581.
Li, X.M., Zheng, K.W., Zhang, J.Y., Liu, H.H., He, Y.D., Yuan, B.F., Hao, Y.H. and Tan, Z. (2015) Guanine-vacancy-bearing G-quadruplexes responsive to guanine derivatives. Proc. Natl. Acad. Sci. USA, 112, 14581-14586.
Liu, J.Q., Xiao, S., Hao, Y.H. and Tan, Z. (2015) Strand-Biased Formation of G-Quadruplexes in DNA Duplexes Transcribed with T7 RNA Polymerase. Angew. Chem. Int. Ed. Engl., 54, 8992-8996.
Wu, R.Y., Zheng, K.W., Zhang, J.Y., Hao, Y.H. and Tan, Z. (2015) Formation of DNA:RNA Hybrid G-Quadruplex in Bacterial Cells and Its Dominance over the Intramolecular DNA G-Quadruplex in Mediating Transcription Termination. Angew. Chem. Int. Ed. Engl., 54, 2447-2451.
Tan, Z., Tang, J., Kan, Z.Y. and Hao, Y.H. (2015) Telomere G-Quadruplex as a Potential Target to Accelerate Telomere Shortening by Expanding the Incomplete End-Replication of Telomere DNA. Curr. Top. Med. Chem., 15, 1940-1946.
Xiao, S., Zhang, J.Y., Wu, J., Wu, R.Y., Xia, Y., Zheng, K.W., Hao, Y.H., Zhou, X. and Tan, Z. (2014) Formation of DNA:RNA Hybrid G-Quadruplexes of Two G-Quartet Layers in Transcription: Expansion of the Prevalence and Diversity of G-Quadruplexes in Genomes. Angew. Chem. Int. Ed. Engl., 53, 13110-13114.
Zheng, K.W., Wu, R.Y., He, Y.D., Xiao, S., Zhang, J.Y., Liu, J.Q., Hao, Y.H. and Tan, Z. (2014) A competitive formation of DNA:RNA hybrid G-quadruplex is responsible to the mitochondrial transcription termination at the DNA replication priming site. Nucleic Acids Res., 42, 10832-10844.
Zhang, J.Y., Zheng, K.W., Xiao, S., Hao, Y.H. and Tan, Z. (2014) Mechanism and Manipulation of DNA:RNA Hybrid G-Quadruplex Formation in Transcription of G-Rich DNA. J. Am. Chem. Soc., 136, 1381-1390.
Zheng, K.W., Xiao, S., Liu, J.Q., Zhang, J.Y., Hao, Y.H. and Tan, Z. (2013) Co-transcriptional formation of DNA:RNA hybrid G-quadruplex and potential function as constitutional cis element for transcription control. Nucleic Acids Res., 41, 5533-5541.
Zhang, C., Liu, H.H., Zheng, K.W., Hao, Y.H. and Tan, Z. (2013) DNA G-quadruplex formation in response to remote downstream transcription activity: long-range sensing and signal transducing in DNA double helix. Nucleic Acids Res., 41, 7144-7152.
Xiao, S., Zhang, J.Y., Zheng, K.W., Hao, Y.H. and Tan, Z. (2013) Bioinformatic analysis reveals an evolutional selection for DNA:RNA hybrid G-quadruplex structures as putative transcription regulatory elements in warm-blooded animals. Nucleic Acids Res., 41, 10379-10390.
Wang, F., Tang, M.L., Zeng, Z.X., Wu, R.Y., Xue, Y., Hao, Y.H., Pang, D.W., Zhao, Y. and Tan, Z. (2012) Telomere- and telomerase-interacting protein that unfolds telomere G-quadruplex and promotes telomere extension in mammalian cells. Proc. Natl. Acad. Sci. USA, 109, 20413-20418.
Xue, Y., Liu, J.Q., Zheng, K.W., Kan, Z.Y., Hao, Y.H. and Tan, Z. (2011) Kinetic and Thermodynamic Control of G-Quadruplex Folding. Angew. Chem. Int. Ed., 50, 8046-8050.
Wang, Q., Liu, J.Q., Chen, Z., Zheng, K.W., Chen, C.Y., Hao, Y.H. and Tan, Z. (2011) G-quadruplex formation at the 3' end of telomere DNA inhibits its extension by telomerase, polymerase and unwinding by helicase. Nucleic Acids Res., 39, 6229-6237.
Chen, C.Y., Wang, Q., Liu, J.Q., Hao, Y.H. and Tan, Z. (2011) Contribution of Telomere G-Quadruplex Stabilization to the Inhibition of Telomerase-Mediated Telomere Extension by Chemical Ligands. J. Am. Chem. Soc., 133, 15036-15044.
Zheng, K.W., Zhang, D., Zhang, L.X., Hao, Y.H., Zhou, X. and Tan, Z. (2011) Dissecting the strand folding orientation and formation of g-quadruplexes in single- and double-stranded nucleic acids by ligand-induced photocleavage footprinting. J. Am. Chem. Soc., 133, 1475-1483.
Wang, Q., Ma, L., Hao, Y.H. and Tan, Z. (2010) Folding equilibrium constants of telomere g-quadruplexes in free state or associated with proteins determined by isothermal differential hybridization. Anal. Chem., 82, 9469-9475.
Liu, J.Q., C.Y. Chen, Y. Xue, Y.H. Hao, & Z. Tan, (2010) G-Quadruplex Hinders Translocation of BLM Helicase on DNA: A Real-Time Fluorescence Spectroscopic Unwinding Study and Comparison with Duplex Substrates. J Am Chem Soc, 132(30): 10521-7.
Zheng, K.W., Z. Chen, Y.H. Hao, & Z. Tan, (2010) Molecular crowding creates an essential environment for the formation of stable G-quadruplexes in long double-stranded DNA. Nucleic Acids Res, 38(1): 327-38.
Chen, Z., Zheng, K. W., Hao, Y. H. and Tan, Z. (2009) Reduced or Diminished Stabilization of the Telomere G-Quadruplex and Inhibition of Telomerase by Small Chemical Ligands under Molecular Crowding Condition. J Am Chem Soc 131(30): 10430-38.
Tang, J., Kan, Z. Y., Yao, Y., Wang, Q., Hao, Y. H. and Tan, Z. (2008) G-quadruplex preferentially forms at the very 3' end of vertebrate telomeric DNA. Nucleic Acids Res 36(4): 1200-8.
Yuan, B. F., Zhuang, X. Y., Hao, Y. H. and Tan, Z. (2008) Kinetics of base stacking-aided DNA hybridization. Chem Commun (Camb)(48): 6600-2.
Wang, F., Zhao, Y., Hao, Y. and Tan, Z. (2008) Identification of low-abundance alternatively spliced mRNA variants by exon exclusive reverse transcriptase polymerase chain reaction. Anal Biochem 383: 307-10.
Xue, Y., Kan, Z. Y., Wang, Q., Yao, Y., Liu, J., Hao, Y. H. and Tan, Z. (2007) Human telomeric DNA forms parallel-stranded intramolecular G-quadruplex in K+ solution under molecular crowding condition. J Am Chem Soc 129(36): 11185-91.
Yao, Y., Wang, Q., Hao, Y. H. and Tan, Z. (2007) An exonuclease I hydrolysis assay for evaluating G-quadruplex stabilization by small molecules. Nucleic Acids Res 35(9): e68.
Kan, Z. Y., Lin, Y., Wang, F., Zhuang, X. Y., Zhao, Y., Pang, D. W., Hao, Y. H. and Tan, Z. (2007) G-quadruplex formation in human telomeric (TTAGGG)4 sequence with complementary strand in close vicinity under molecularly crowded condition. Nucleic Acids Res 35(11): 3646-53.
Zhuang, X. Y., Tang, J., Hao, Y. H. and Tan, Z. (2007) Fast detection of quadruplex structure in DNA by the intrinsic fluorescence of a single-stranded DNA binding protein. J Mol Recognit 20(5): 386-91.
Yuan, B. F., Xue, Y., Luo, M., Hao, Y. H. and Tan, Z. (2007) Two DNAzymes targeting the telomerase mRNA with large difference in Mg2+ concentration for maximal catalytic activity. Int J Biochem Cell Biol 39(6): 1119-29.
Kan, Z. Y., Yao, Y., Wang, P., Li, X. H., Hao, Y. H. and Tan, Z. (2006) Molecular crowding induces telomere G-quadruplex formation under salt-deficient conditions and enhances its competition with duplex formation. Angew Chem Int Ed Engl 45(10): 1629-32.
Zhao, Y., Zeng, Z. X., Kan, Z. Y., Hao, Y. H. and Tan, Z. (2005) The folding and unfolding kinetics of the i-motif structure formed by the C-rich strand of human telomere DNA. Chembiochem 6(11): 1957-60.
Zeng, Z. X., Zhao, Y., Hao, Y. H. and Tan, Z. (2005) Tetraplex formation of surface-immobilized human telomere sequence probed by surface plasmon resonance using single-stranded DNA binding protein. J Mol Recognit 18(3): 267-71.
Shao, L., Li, Q. H., Wang, J. and Tan, Z. (2005) Fragmentation and rapid shortening of telomere in HeLa cells in the early phase of hydroxyl radical-induced apoptosis. Cancer Biol Ther 4(3): 336-41.
Li, W. G., Li, Q. H. and Tan, Z. (2005) Epigallocatechin gallate induces telomere fragmentation in HeLa and 293 but not in MRC-5 cells. Life Sci 76(15): 1735-46.
Li, W. G., Li, Q. H. and Tan, Z. (2005) Detection of telomere damage as a result of strand breaks in telomeric and subtelomeric DNA. Electrophoresis 26(3): 533-6.
Zhao, Y., Kan, Z. Y., Zeng, Z. X., Hao, Y. H., Chen, H. and Tan, Z. (2004) Determining the folding and unfolding rate constants of nucleic acids by biosensor. Application to telomere G-quadruplex. J Am Chem Soc 126(41): 13255-64.
Yuan, B. F., Hao, Y. H. and Tan, Z. (2004) Universal sensing strategy for the detection of nucleic acid targets by optical biosensor based on surface plasmon resonance. Clin Chem 50(6): 1057-60.
Shao, L., Li, Q. H. and Tan, Z. (2004) L-carnosine reduces telomere damage and shortening rate in cultured normal fibroblasts. Biochem Biophys Res Commun 324(2): 931-6.
Hao, Y. H. and Tan, Z. (2002) The generation of long telomere overhangs in human cells: a model and its implication. Bioinformatics 18(5): 666-71.
Tan, Z. (2001) Simulated shortening of proliferation-restricting telomeres during clonal proliferation and senescence of human cells. Exp Gerontol 36(1): 89-97.
Hao, Y. H. and Tan, Z. (2001) Telomeres at the chromosome X(p) might be critical in limiting the proliferative potential of human cells. Exp Gerontol 36(10): 1639-47.